Isolation, Identification and Antibiogram Pattern of Avibacterium Paragallinarum from Japanese Quails
نویسنده
چکیده
A total of 53 trachea, air sacs, lung, and infra orbital sinus exudates were collected from 5 commercially reared Japanese quail farms showing typical symptoms of infectious coryza. Avibacterium paragallinarum was isolated and identified using selective media and by biochemical identification methods. Biochemical identification tests such as satellitism, IMViC tests and carbohydrate fermentation tests were performed. In total, eight A. paragallinarum isolates were isolated and identified from the field samples and the species were confirmed with the species specific PCR, which gives the amplicon size of 500bp. Antibiogram of A. paragallinarum showed 100% resistance for Ampicillin, neomycin, Pefloxacin, co-trimaxazole, furazolidone, streptomycin, Cephalexin and Amikacin with 90% for Gentamycin and 70% for oxytetracycline.
منابع مشابه
Isolation and identification of Avibacterium paragallinarum, the causal agent of infectious coryza (IC) from layer chickens in Bangladesh
This study was carried out for the isolation and identification of Avibacterium paragallinarum, the etiological agent of infectious coryza (IC). Pathological changes were also investigated that occurred in organs in layer chickens obtained from some selected areas of Bangladesh. A nasal swabs (n=30) from dead chickens and four swabs from live chickens were collected aseptically. The organisms f...
متن کاملRetrospective Detection of Avibacterium Paragallinarum Serovar B in Egg Yolk Materials by PCR
Avibacterium paragallinarum is Gram-negative bacteria that cause infectious coryza (IC), an acute respiratory disease of chickens. Despite vaccination of Layer and breeder farms of Iran against IC, they are still experiencing the disease clinically and there is no knowledge of serotypes prevalence of this bacterium in this country. This study designed to determine serovar identity by molecular ...
متن کاملDraft Genome Sequence of the Virulent Avibacterium paragallinarum Serotype A Strain JF4211 and Identification of Two Toxins
Avibacterium paragallinarum is an important pathogen of chicken livestock causing infectious coryza. Here, we report the draft genome sequence of the virulent A. paragallinarum serotype A strain JF4211 (2.8 Mbp and G+C content of 41%) and the two toxin operons discovered from the annotation of the genome.
متن کاملReclassification of Pasteurella gallinarum, [Haemophilus] paragallinarum, Pasteurella avium and Pasteurella volantium as Avibacterium gallinarum gen. nov., comb. nov., Avibacterium paragallinarum comb. nov., Avibacterium avium comb. nov. and Avibacterium volantium comb. nov.
This paper describes a phenotypic and genotypic investigation of the taxonomy of [Haemophilus] paragallinarum, Pasteurella gallinarum, Pasteurella avium and Pasteurella volantium, a major subcluster within the avian 16S rRNA cluster 18 of the family Pasteurellaceae. An extended phenotypic characterization was performed of the type strain of [Haemophilus] paragallinarum, which is NAD-dependent, ...
متن کاملAmplified 16S ribosomal DNA restriction analysis for identification of Avibacterium paragallinarum.
A molecular technique based on the restriction fragment length polymorphism of the 16S ribosomal genes amplified by a polymerase chain reaction (PCR), referred to as amplified 16S ribosomal DNA restriction analysis (ARDRA), was designed to identify 19 Avibacterium paragallinarum strains isolated from infraorbital sinus and nasal turbinate bone samples of broiler chickens, breeders, and laying h...
متن کامل